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Next generation sequencing

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The Ramaciotti Centre for Genomics is a long-established, experienced provider of next generation sequencing services. We have state-of-the-art short and long read NGS technologies with scalable capacity. Our expert team of scientists have extensive experience and we always provide our clients data of the highest quality.Ìý

All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

The range of NGS services that we offer is extensive, if there is a technique or technology you are interested in that is not listed on the service menu on the right,Ìýcontact usÌýas we may be able to offer an alternative solution.

  • We can deliver the complete genome sequencing package using our suiteÌýofÌýIllumina andÌýOxford Nanopore Technologies next-generation sequencers. Whether you are sequencing a bacterial, human, or other genome, our experienced team can help you design your project and deliver high quality data.ÌýContact usÌýfor a quote or assistance with your project design.Ìý

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Service Genome type - Description
    Illumina DNA Prep All genomes
    Illumina DNA PCR-free Prep All genomes
    Oxford Nanopore - long readÌý All genomes/Full length plasmid
    TraDIS sequencing Sequencing of transposon introduced mutations

  • Complete plasmid sequencing for constructs between 2kb and 25kb.

    Uses Oxford Nanopore Technologies for full-length long read sequencing to generate high consensus accuracy.

    No primers needed.

    The cost isÌý$35/plasmid. For UNSW internals: no quote is required, simply provide account codes in the submission form; For externals to UNSW: email us to request a quote.

    Ìý

    Submit your order via theÌý.

    Place the samples into the "ONT Plasmid" reception box in the Sanger freezer at Ramaciotti Centre entryÌýon level 2 of E26 UNSW

    ORÌýÌý

    Send samples to ourÌýparcel delivery address. Ìý

    For service specific sample submission criteria, seeÌýWhole Plasmid Sequencing using Oxford Nanopore Requirements.

    Ìý

    Turnaround time isÌý4-10 business days after successful QCÌýof submitted samples.

    The ONT sequencing data output will be:Ìý

    • Analysis using theÌýÌýworkflow; output FASTA sequence and report on the assembled plasmid. Ìý
    • Raw ONT data Ìý
    • Run summary: report from the GridIONÌýor PromethIONÌýrun

    Do not need full-length plasmid sequencing? Learn about ourÌýSanger SequencingÌýservices.

  • Exome, panel or targeted sequencing focuses on specific areas of interest reducing sequencing costs and simplifying analysis. We have experience with aÌýwide range of methods for DNA capture, some of these are listed in the table below.ÌýContact usÌýto discuss your project and canÌýwill advise you on the best approach.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Type Capture product
    Exome Core or comprehensiveÌýÌý
    Targeted / panel Custom or pre-designed
    TargetedÌý Amplicon - Illumina or Oxford Nanopore
    TargetedÌý CRISPR
    TraDIS sequencing Whole genome sequencing of transposon introduced mutations

  • The Ramaciotti Centre for Genomics offers four sequencing-based services for the study of the epigenome:Ìý

    • ChIP-seq - analysisÌýof histone modifications and protein-DNA interactions on a genome-wide scale.
    • Methyl-seq - genome-wide or targeted DNA methylation analysis.
    • Nucleosome sequencingÌý(client prepared libraries).
    • Whole or targeted DNA and RNA methylation by long read sequencing.Ìý

    Contact usÌýfor further information on these services or for a quote. Please note we also offer methylation-analysis by array.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

  • We have been providing RNA sequencing services to the research community since 2010 and haveÌýexperience using a wide variety of RNA sequencing protocols allowing us to tailor our services to yourÌýproject. We provide excellentÌýproject support, assisting you with design and approach, and we always deliverÌýhigh quality data at a competitive price.

    Contact usÌýto discuss your project and we can advise you on the best approach.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.
    Service Description
    mRNA seq Coding only, stranded
    Total RNA seq* Coding & non-coding, stranded
    Total RNA seq - low input Coding & non-coding, stranded - lower starting amount
    Total RNA seq - depleted Coding & non-coding, stranded - client depleted
    Small RNA seq Small RNA up to 36nt
    Direct RNA sequencing Oxford Nanopore -Ìýlong read
    RNA capture seq Targeted enrichment of RNA regions of interest

    * Species supported: human, mouse, rat, plant andÌýbacteria. Globin reduction services also available.

  • Single-cell sequencing is more accessible than ever. We offer single-cell RNA sequencing through variousÌýmicrofluidic-free methods, catering to both low- and high-throughput requirements.

    Please contact us to explore options that best meet your specific needs or click here for more information on our single-cell sequencing service.

  • The Ramaciotti Centre for Genomics provides a range of targeted next-generation sequencing servicesÌýthat enableÌýthe taxonomic profiling of mixed communities. These methods useÌýprimers designed to target and capture taxonomic regions, followed by next-generation sequencing.ÌýÌýTargeted sequencing approaches to study the microbiome are oftenÌýknown as diversity profiling orÌýamplicon, metabarcoding orÌýtag sequencing.

    Costs start atÌý$37/sampleÌýfor our standard microbiome sequencing services.Ìý

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

    Microbiome sequencing services -16S/18S/ITS

    We haveÌýa range of pre-optimised gene region targets ready to go, refer to the table below. The workflowÌýincludes PCR to incorporate unique barcodes for each sample, pooling of samples into a single library tube, and sequencing on an Illumina sequencer.Ìý
    Ìý

    Target Gene region Primers Used by*
    Bacteria 16S V1-V3 27f - 519r AM, BASE
    Ìý 16S V3-V4 341f-805r Ìý
    Ìý 16S V4 515f - 806r EMPÌý
    Ìý 16S full length 27f - 1492r ONT - rapid 16S kitÌý
    Eukaryote 18S V4 18SV4F_18SV4R AM, MM
    Ìý 18S V9 1391f - EukBr AM, BASE, EMP
    Eukaryote - Fungi ITS1 ITS1F-ITS2 EMP
    Ìý ITS2 fITS7-ITS4 Ìý
    Archea A16S A2f - 519r AM, BASE
    * AM -ÌýÌýproject, MM -ÌýÌýproject, BASE -ÌýÌýproject, EMP -ÌýÌý.

    The AM, BASE and MM projects areÌý.

    Custom target sequencing services

    If you would like to target genes not listed above we can offer a custom short read approach based on aÌýÌýdesign. For this you would perform an initial round of PCR using a pair of gene/locus specific primers which includeÌýIllumina adapter overhangs and then submitÌýthe purified product to us for sequencing. Multiple gene targets can be pooled in a sequencing run if they are size compatible.ÌýCustom amplicons can also beÌýsequencedÌýusing Oxford Nanopore longÌýread technology.Ìý

    We would be happy to advise you on our custom target sequencing, pleaseÌýcontactÌýus.

  • NGS-based metagenomic sequencing can comprehensively sequence and analyse all genetic material in your complex sample. This methodÌýcan detect very low abundance members of a microbial community that may be missed by other methods providingÌýa means to study microorganisms that are otherwise difficult to analyse.ÌýContact usÌýfor a quote or assistance with the design of your project.

    All sequencing performed by the Ramaciotti Centre for Genomics is carried out in our ISO/IEC 17025 accredited laboratories.

  • Our Client Prepared Library service gives you the option of preparingÌýyour own library pools in-house and sending them to us for sequencing. Some of the benefits of outsourcing your sequencing to us are:

    Flexibility

    You can choose from any sequencerÌýin ourÌýfleetÌýofÌýsequencingÌýinstruments, allowing you to scale up or down depending on your current needs.ÌýÌý

    Quality assurance

    NATA Accredited toÌýÌýensuringÌýthat you receive data of theÌýhighest quality data.

    Cost effective

    Competitive pricingÌýwith access to our discount reagent costs.Ìý

    Fast turnaround time

    3-10Ìýbusiness days for standard kit types.

    Expertise

    We haveÌý>16 years of experience in NGS service provision.Ìý

    No ongoing costs

    You avoid the ongoing annual service contracts required when you own your own sequencer.Ìý
    Ìý

    Contact usÌýfor more information or for a quote.